Los Alamos National Laboratory
The Interagency Biological Restoration Demonstration (IBRD) program has a mission to identify and demonstrate applied technology solutions that enable recovery and restoration efforts and further research in determining the persistence and viability of Bacillus anthracis if released in a wide-area urban environment. CSS worked under subcontract to the Los Alamos National Laboratory (LANL) in support of the Defense Threat Reduction Agency (DTRA) to study the fate of Bacillus thuringiensis var. Kurstaki (Btk) used in gypsy moth eradication programs that involve wide-area spraying of Btk in Fairfax County, VA and metropolitan Seattle, WA. CSS provided all of the services necessary to collect vegetation, soil, water (runoff or other non-drinking water samples), surface, HVAC filters, and aerosol samples at specific locations in both areas. In support of these efforts, CSS placed aerosol sampling units on the upwind and downwind sides of filters in the HVAC plenum and near external fresh-air uptakes. CSS employees also collected swipe samples from horizontal surfaces within the HVAC system, immediately downwind of HVAC intakes and vents, and electrostatically-active surfaces (e.g., computer screens). Employees collected these samples before and immediately after spraying had occurred for 2 weeks, then every week for an additional 10 weeks.